سپیده احمدی – گروه زیست شناسی، دانشکده علوم پایه، دانشگاه زابل، زابل، ایران
حسین کمال الدینی – گروه زیست شناسی، دانشکده علوم پایه، دانشگاه زابل، زابل، ایران
فاطمه حدادی – گروه زیست شناسی، دانشکده علوم پایه، دانشگاه زابل، زابل، ایران
The combination of nanotechnology with chemistry, biology, physics, and medicine for the development of ultrasensitive
detection and imaging methods in the analytical or biological sciences is becoming increasingly important in modern
science(2,3). Gold nanoparticles are nontoxic, biocompatible nanomaterials that can be either obtained from commercial
sources or conveniently produced in laboratories(1). Electrical and optical properties of gold nanoparticles and the
penetration of its magnetic field into the cells could be come into consideration for detection and diagnosis of genetic
diseases, and biosensor studies. In recent years gold nanoparticles has been used to detect cancer, pathogenic disease and bacteria(4). Since combination of PCR technique and gold nanoparticles could be applied in diagnosis detection, therefor possibility of interaction between PCR components and gold nanoparticles is important to be studied.
In this study, effect of PCR ingredients like PCR buffer and MgCl2 and their concentrations, on sedimentation of gold
nanoparticles was investigated.
Materials and Methods
AuNPs was prepared by sodium citrate. Different concentrations of PCR buffer (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 and 4.5) with
constant concentration of 50 μl gold nanoparticles were applied. Final volume of all reactions was adjusted to 55 μl with
deionized distilled water. Another experiment was also performed by application of the same concentrations for MgCl2.
Moreover effect of PCR Buffer and MgCl2 combination was evaluated using constant PCR buffer concentration (2μL) and
various concentrations of MgCl2.
Results showed that gold nanoparticles color was changed at a concentration of 2.5 μl for PCR buffer and 2 μl of MgCl2.
Additionally the combination of PCR buffer and MgCl2 changed the color of AuNPs at 0.5 μl MgCl2.
According to the results, the precipitation of gold nanoparticles could be influenced by different concentrations of the PCR
component. Hence to describe the results of studies using combination of PCR and gold nanoparticles this note should be considered.
Key words: Gold nanoparticles, Sodium citrate, PCR buffer, MgCl2